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. 2016 Apr 15;5(4):e70. doi: 10.1038/cti.2016.16

Figure 5.

Figure 5

PD-1 expression on Treg, CD4 and CD8 T cells in HPV-positive and -negative patients. (a) The percentages of PD-1-positive T cells were quantified as shown in Figure 3 for Tregs, conventional CD4s and CD8s. Means for the HPV-positive and -negative groups are depicted as red and blue lines, respectively, in the plots; P-values are from non-parametric repeated-measures (blood, TIL) models of each cell type. ((+)=HPV positive, N=14), ((o)=HPV−, N=11) P-values for HPV+ vs − were not significant. (b) MFI: Tregs, convention CD4s and CD8 T cells were gated on as individual populations and evaluated for PD-1 MFI in the blood and TIL of head and neck cancer patients. Because of physical changes in our flow cytometer over the course of the study, the fold-change in MFI of TIL relative to blood was used to normalize the MFI data set and is shown for each subset of T cells. As in Figure 4, in a subset of samples with consistent cytometer settings there was significant differences in MFI in the Treg population (P=0.0313) and conventional CD4 population (P=0.0313), as well as the CD8 population (P=0.002) when the TIL were compared with blood (data not shown).