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. 2016 Apr 15;5(4):e70. doi: 10.1038/cti.2016.16

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Copyright © 2016 Australasian Society for Immunology Inc.

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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OX40 is preferentially expressed in the tumor compartment of head and neck squamous cell carcinoma. Multiplex fluorescent immunohistochemistry was performed on two SCCHN tumor specimens (a, b). Five markers were evaluated, including PD-1 (red), OX40 (yellow), FoxP3 (green), CD3 (magenta) and DAPI (blue). Arrows are pointing to cells with the following phenotypes. Yellow arrow: OX40⁺CD3⁺FoxP3⁺ cell; red arrow: OX40⁺CD3⁺FoxP3⁻ cell; magenta arrow: OX40⁻CD3⁺FoxP3⁺ cell; white arrow: OX40⁺CD3⁺PD-1⁺ cell. The insets points to cells co-expressing OX40 (yellow) and PD-1 (red).