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A p21-activated kinase (PAK) catalytic domain (CD) swapping experiment. (a) Schematic diagram of PAK constructs. (b) HeLa cells were transfected with plasmids encoding wild-type PAK or CD-swapped PAK (R1K3 or R3K1). After 24 h, cells were exposed to the indicated inhibitor (20 μM) for 2 h before stimulation with 0.4 M sorbitol. PAK1 activation was monitored by immunoblotting for pPAK1T423. RD, regulatory domain. All data are representative of at least three independent experiments.
