Fig. 5.
CGRP inhibits THP-1 mediated OPN secretion as elicited by high levels of LPS only. THP-1 macrophage-like cells co-cultured with MG-63 osteoblasts were stimulated with LPS or UHMWPE particles. The effects of CGRP (10−8 M) treatment on co-cultured cells were analyzed. OPN secretion, as normalized to the unstimulated control, of co-cultures and controls activated by high levels of LPS (100 ng/ml) for a 6 h and b 24 h of incubation or activated by c UHMWPE (cell-to-particle ratio of 1:500) for 6 h of incubation as compared to the respective OPN fold secretion of activated cells treated with the neuropeptide CGRP. *p < 0.05; **p < 0.01; ***p < 0.001