Fig. 7.

ALP expression and activity are marginally influenced by UHMWPE particles and LPS in co-cultured osteoblasts. THP-1 macrophage-like cells co-cultured with MG-63 osteoblasts were stimulated with LPS (100 ng/ml) or UHMWPE particles (cell-to-particle ratio of 1:500). a Relative ALP mRNA expression by both co-cultures and controls as compared to the housekeeping gene GAPDH at 24 h of incubation is exemplarily shown. b ALP activity [U/ml] in both co-cultures and controls as per 10 μg of total protein at 24 h of incubation is exemplarily shown