Table 1.
MG-63 osteoblasts co-cultured with THP-1 macrophages under virtually osteolytic conditions treated with CGRP
| Incubation time | Stimulation | Treatment | |||
|---|---|---|---|---|---|
| Negative Control | LPS (10 pg/ml) | LPS (100 ng/ml) | UHMWPE (1:500) | ||
| 6 h | MG-63 Control | MG-63 Control | MG-63 Control | MG-63 Control | Control |
| MG-63 Control | MG-63 Control | MG-63 Control | MG-63 Control | CGRP (10−8 M) | |
| MG-63 + THP-1 | MG-63 + THP-1 | MG-63 + THP-1 | MG-63 + THP-1 | Control | |
| MG-63 + THP-1 | MG-63 + THP-1 | MG-63 + THP-1 | MG-63 + THP-1 | CGRP (10−8 M) | |
| 24 h | MG-63 Control | MG-63 Control | MG-63 Control | MG-63 Control | Control |
| MG-63 Control | MG-63 Control | MG-63 Control | MG-63 Control | CGRP (10−8 M) | |
| MG-63 + THP-1 | MG-63 + THP-1 | MG-63 + THP-1 | MG-63 + THP-1 | Control | |
| MG-63 + THP-1 | MG-63 + THP-1 | MG-63 + THP-1 | MG-63 + THP-1 | CGRP (10−8 M) | |
| 48 h | MG-63 Control | MG-63 Control | MG-63 Control | MG-63 Control | Control |
| MG-63 Control | MG-63 Control | MG-63 Control | MG-63 Control | CGRP (10−8 M) | |
| MG-63 + THP-1 | MG-63 + THP-1 | MG-63 + THP-1 | MG-63 + THP-1 | Control | |
| MG-63 + THP-1 | MG-63 + THP-1 | MG-63 + THP-1 | MG-63 + THP-1 | CGRP (10−8 M) | |
THP-1 macrophage-like cells, co-cultured with MG-63 osteoblast-like cells, were subjected to various virtually osteolytic stimuli (negative control, LPS concentrations of 10 pg/ml and 100 ng/ml, and UHMWPE with a cell-to-particle ratio of 1:500) and neuropeptide treatment (control, CGRP) for 6, 24 and 48 h of incubation