Fig. 7.

Hypoxia enhanced Prdx1-induced IL-6 and TNF-α production. Human monocytes, cultured under normoxia or hypoxia condition for 18 h, were incubated either with solvent control or rPrdx1 (11 μg/mL) for another 24 h in normoxia before the supernatants were harvested and analyzed for IL-6 (a) and TNF-α (c) levels. b The culture supernatants from human monocytes, under normoxia or hypoxia condition for 18 h and then followed by another 24 h in normoxia, were harvested and analyzed for the Prdx1 levels. d Human monocytes, cultured under normoxia or hypoxia condition for 6 h, were further incubated in normoxia for 24 h before surface TLR4 and CD14 expressions were analyzed. Data represented means and SEM for triplicate samples. *P < 0.05, **P < 0.01, and ***P < 0.001