Fig. 3.

VEGF-B restored H/R-induced alterations of H9C2 cell morphology and cell apoptosis. a Cell apoptosis was detected using DAPI. b Cell apoptosis was detected using Annexin V/PI staining, n = 6. c Quantitative analysis of cell apoptosis in (b). *P < 0.05 vs. 15 % FBS-treated cells; ^# P < 0.05 vs. H/R-treated cells; ^@ P < 0.05 vs. 0.1 ng/ml VEGF-B-treated cells; ^$ P < 0.05 vs. 1.0 ng/ml VEGF-B-treated cells; ^& P < 0.05 vs. 10 ng/ml VEGF-B-treated cells; ^{^} P > 0.05 vs. 20 ng/ml VEGF-B-treated cells, n = 6. d VEGF-B reduced LDH levels. *P < 0.05 vs. 15 % FBS-treated cells; ^# P < 0.05 vs. H/R-treated cells; ^@ P < 0.05 vs. 0.1 ng/ml VEGF-B-treated cells; ^$ P < 0.05 vs. 1.0 ng/ml VEGF-B-treated cells; ^& P < 0.05 vs. 10 ng/ml VEGF-B-treated cells; ^{^} P > 0.05 vs. 20 ng/ml VEGF-B-treated cells, n = 6. e Bcl-2 and Bax expression was detected using western blot, as described in the Materials and methods section (e), and quantified via normalization to a-tubulin (f–h). *P < 0.05 vs. 15 % FBS-treated cells; ^# P < 0.05 vs. H/R-treated cells; ^$ P < 0.05 vs. 1.0 ng/ml VEGF-B-treated cells; ^& P < 0.05 vs. 10 ng/ml VEGF-B-treated cells, n = 6