Fig. 6.

VEGF-B inhibited H9c2 cell apoptosis by inhibiting H/R-induced autophagy. a Cell apoptosis was detected using Annexin V/PI staining, n = 6. b Quantitative analysis of cell apoptosis in (a). c Representative images showing LC3 staining in different groups of H9c2 cells infected with Ad-GFP-RFP-LC3 for 48 h. c LC3-II/I expression was detected using western blot, as described in the “Methods” section (a), and quantified using normalization to a-tubulin (d). *P < 0.05 vs. 15 % FBS-treated cells; ^# P < 0.05 vs. H/R-treated cells; ^@ P < 0.05 vs. 20 ng/ml VEGF-B-treated cells; ^$ P > 0.05 vs. H/R-treated cells, n = 6