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. 2016 May 3;9:257. doi: 10.1186/s13071-016-1518-1

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© Zhou et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — SDS-PAGE and immunoblot analysis of recombinant and native BcMSA1 and BcSA1. a The 10 % SDS-PAGE stained with Coomassie blue: recombinant BcMSA1 fused with GST (lane 1) and GST (lane 2). Western blot analysis of recombinant protein: recombinant BcMSA1 (lane 3) and GST (lane 4) probed with B. canis-infected dog serum; b Western blot analysis of native BcMSA1. B. canis-infected erythrocyte lysate (lane 1) and normal canine erythrocyte lysate (lane 2) probed with anti-rBcMSA1 mouse serum; c The 10 % SDS-PAGE stained with Coomassie blue: recombinant BcSA1 fused with GST (lane 1), recombinant BcSA1 fused without GST (lane 2) and GST (lane 3). Western blot analysis of recombinant protein: recombinant BcMSA1 (lane 4), recombinant BcSA1 fused without GST (lane 5) and GST (lane 6) probed with B. canis-infected dog serum; d Western blot analysis of native BcMSA1. The plasma from a dog infected with B. canis (lane 1) and a normal dog (lane 2) probed with anti-rBcMSA1 mouse serum