. 2016 May 4;16:124. doi: 10.1186/s12906-016-1105-1

Table 4.

Effect of sub-inhibitory concentrations of Nauclea latifolia stem bark extract on the activities of first line antibiotics against Gram-negative MDR bacteria

Antibiotics and concentrations of extract		Bacterial strains and MIC values (μg/mL)						PBSS (%)
Antibiotics and concentrations of extract		K. pneumoniae K24	P. stuartii NAE16	E. coli AG100	E. aerogenes EA289	P. aeruginosa PA124	E. aerogenes CM64	PBSS (%)
TET	0	64	64	16	8	16	32	-
	MIC/2	64(1)^I	64(1)^I	8(2)^S	8(1)^I	16(1)^I	16(2)^S	33.33
	MIC /5	64(1)^I	64(1)^I	16(1)^I	8(1)^I	16(1)^I	16(2)^S	16.67
NOR	0	≥64	1	≤0.25	-	128	2(1)^I	-
	MIC/2	≥64	1(1)^I	≤0.25	-	128 (1)^I	2(1)^I	0
	MIC /5	≥64	1(1)^I	≤0.25	-	128(1)^I	2(1)^I	0
STR	0	2	32	≥256	64	64	16	-
	MIC/2	2(1)^I	32(1)^I	≥256	32(2)^S	64(1)^I	8(2)^S	16.67
	MIC /5	2(1)^I	32(1)^I	≥256	64(1)^I	64(1)^I	16(1)^I	0
KAN	0	8	8	8	32	64	≤2	-
	MIC/2	8(1)^I	8(1)^I	4(2)^S	16(2)^S	64(1)^I	≤2	33.33
	MIC /5	8(1)^I	8(1)^I	8(1)^I	32(1)^I	64(1)^I	≤2	16.67
CHL	0	16	8	8	512	64	512	-
	MIC/2	16(1)^I	4(2)^S	8(1)^I	512(1)^I	64(1)^I	512	16.67
	MIC /5	16(1)^I	8(1)^I	8(1)^I	512(1)^I	64(1)^I	512	0
ERY	0	128	16(1)^I	64	128	128	256	-
	MIC/2	128(1)^I	16(1)^I	64(1)^I	128(1)^I	128(1)^I	128(2)^S	16.67
	MIC /5	128(1)^I	16(1)^I	64(1)^I	128(1)^I	128(1)^I	256(1)^I	0
AMP	0	-	-	128	-	-	-	-
	MIC/2	-	-	256(2)^I	-	-	-	0
	MIC /5	-	-	256(2)^I	-	-	-	0

(-) : >256 μg/ml; (): fold decrease in MIC values of the antibiotics after association with plants extract; S: Synergy, I: Indifference; A: antagonism, Antibotics (CHL chloramphenicol, AMP ampicillin, KAN kanamycin, NOR norfloxacin, ERY erythromycin, TET tetracycline, STR streptomycin); PBSS percentage of bacteria strain on which synergism has been observed