Table 3.
Comparison of residual ATP detected by the direct sponge method versus level of residual protein and viable count in colonoscope instrument channels
| Sponge sampleb (N = 3) | Flush samplec (N = 3) | |||
|---|---|---|---|---|
| Test parametera | RLUs (Std) | Protein μg/cm2 (Std) | E. faecalis Log10 cfu/cm2 (Std) | P. aeruginosa Log10 cfu/cm2 (Std) |
| Positive control Soiled but no cleaning |
9747.00 (436.48) | 335.4 (65.55) | 6.782 (0.212) | 6.669 (0.192) |
| Partial clean Soiled, 50 mL water flush and no brushing |
6601.00 (1684.00) | 3.99 (3.16) | 5.254 (0.142) | 4.476 (0.253) |
| Full cleaning Soiled, cleaned pre-HLD |
29.00 (36.39) | 0.23 (0.07) | 0.787 (0.364) | 1.608 (0.269) |
| Negative control Soiled, cleaned post-HLD and storage |
14.00 (21.66) | 0.04 (0.07) | 0.025 any organism (0.090) | |
aData for each test parameter represents the average and standard deviation (Std) of three replicate endoscope tests (N = 3)
bATP was measured as relative light units (RLUs)
cThe protein and viable counts were done on a parallel set of colonoscopes that were soiled in the same manner and were harvested using the flush method of sample collection