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. Author manuscript; available in PMC: 2016 May 4.
Published in final edited form as: Photochem Photobiol. 2014 Jun 3;90(5):1034–1042. doi: 10.1111/php.12288

Figure 4.

Figure 4

The propensity of TMR-Rn to disrupt photo-oxidized RBCs is optimal for n=9. A, B) In the dark, TMR-Rn peptides lyse membrane of RBCs photo-oxidized by Rose Bengal. RBCs incubated with 1 μM RB were irradiated followed by addition of 1 μM and 5 μM TMR-Rn peptide in the dark. This is represented as light conditions. Alternately, the ability if Rn CPPs to disrupt un-oxidized (no irradiation but RB added) at 1 μM and 5 μM is also represented and referred to as dark conditions. High arginine content in CPPs resulted in dark lysis of un-oxidized RBC membranes but also enhanced disruption of photo-oxidized RBCs membranes. Hemolysis was measured at 450 nm and plotted against number of arginine residues on the CPP. The data shown represents the average of 3 experiments and the corresponding standard deviations. C) Net lysis of RBCs caused by Rn CPPs at 1 μM, 3 μM and 5 μM is shown here. The lysis caused by CPPs under dark conditions was subtracted from lysis caused by CPPs of photo-oxidized RBCs. 100% hemolysis was determined by addition of Triton X-100 to RBCs.