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. Author manuscript; available in PMC: 2016 Sep 7.
Published in final edited form as: Nature. 2016 Mar 7;531(7594):395–399. doi: 10.1038/nature17163

Extended Data Figure 3. Purification of an active translocation complex.

Extended Data Figure 3

a, Scheme of the purification protocol. b, Elution of the G. thermodenitrificans SecYE – B. subtilis SecA-OAIns complex from a Superdex200 column during the last chromatography step. c, Non-reducing SDS-PAGE analysis of samples taken during the purification procedure and of fractions indicated with red numbers in b. Lane 1, molecular weight markers. Lane 2, sample analyzed after IMAC. Lane 3, sample after cleavage of the GFP tag. Lane 4, sample after anion exchange chromatography (MonoQ).