Fig. 2.

Histogram of novel FXR binding sites within known FXR target genes, Nr0b2 and Ostb. The y-axis displays the peak value and the x-axis shows the chromosomal location of the gene. The top panel (displayed in red) represents FXR binding in liver, and the bottom panel (displayed in blue) represents FXR binding in intestine. Genes that are displayed above the chromosome scale are oriented in the sense directions (right arrow), and genes that are displayed below the chromosome scale are oriented in the antisense direction (left arrow). The threshold is set at peak value >20. (A) The Nr0b2 gene is located on chromosome 4 (Chr4): 133109305-133112451 and is oriented in the sense direction in this figure. FXR binds at two locations around the Nr0b2 gene in intestine, promoter region (peak value = 124) and 3′ end of the gene (peak value = 381). FXR binds at four locations of the Nr0b2 gene in liver, two in the promoter and two at the 3′ end (peak values are 69, 258, 498, and 49, respectively). (B) The Ostb gene is located at Chr9: 65260560-65270580 and is oriented in the antisense direction. In intestine, FXR binds at four locations near the Ostb gene, with three sites within the promoter (peak value = 572, 27, and 210) and one located at the 3′ end (peak value = 132). In liver, FXR binds once within the promoter (peak value = 271) and once at the 3′ end (peak value = 44). Novel FXR sites found at the 3′ end of both Nr0b2 and Ostb gene have not been reported and are indicated by an asterisk.