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Glycosidase digestion pattern of hGLUT1 expressed in yeast. Lane 1, protein marker; lane 2, undigested; lane 3, endoglycosidase H (EndoH); lane 4, peptide: N-glycosidase F (PNGase). No difference in bandwidth or band density was observed between EndoH and PNGase F digestion, thus supporting lack of glycosylation of hGLUT1 expressed in yeast.
