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. 2016 Apr 11;113(17):4723–4728. doi: 10.1073/pnas.1519128113

Fig. 3.

Fig. 3.

Minor alterations of dNTP pools further elevate mutation rates in MMR-deficient cells. (A and B) Amount of each dNTP (A) and mutation rates (B) in the WT, msh2Δ, rnr1-Y285F, and msh2Δ rnr1-Y285F yeast strains. (C) Amount of each dNTP normalized to the total NTP pool in untreated HCT116 cells (control), HCT116 cells incubated in the presence of 50 μM thymidine and 20 μM deoxyadenosine for 20 h (20 h), and in HCT116 cells incubated in the presence of 50 μM thymidine and 20 μM deoxyadenosine for 20 h, after which an additional 50 μM thymidine and 20 μM were added. dNTP pools were measured after 1 h (+1 h) and after 4 h (+4 h). (D) Flow cytometry histograms of the HCT116 cells used for dNTP pool measurements in C. (E) Mutation frequencies and PDs of the HCT116 cells incubated in the presence or absence of 50 μM thymidine (dT) and 20 μM deoxyadenosine (dA).