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. 2016 Apr 11;113(17):4723–4728. doi: 10.1073/pnas.1519128113

Fig. S2.

Fig. S2.

In vitro dNTPase activity assays of SAMHD1 protein and the four mutants over a time course of 60 min. All reactions were performed by using the same sample of each protein. Reactions with a total volume of 300 μL [10 mM Tris⋅HCl (pH 7.5), 50 mM NaCl, 5 mM MgCl2, 1 mM GTP, 1 mM dNTP, and 0.5 μM SAMHD1] were incubated at 25 °C. Aliquots were collected at 0, 15, 30, 45, and 60 min, and the dN products were quantified. Lines were fitted by using the linear regression function in GraphPad Prism.