Table S1.
ΔG, m value from CD, chevron, fluorescence, and HX
| Experiment | Location | Buffer, H2O/D2O | ΔGfit, kcal·mol−1 | ΔGHX,0Mmeasure, kcal·mol−1 | m, kcal·mol−1·M−1 |
| Kinetic (chevron) | Global | 100/0 | 6.96 ± 0.15 | — | 1.01 ± 0.02 |
| CD | Global | 100/0 | 6.96 ± 0.02 | — | 1.00 ± 0.02 |
| CD | Global | 20/80 | 7.38 ± 0.32 | — | 1.00 ± 0.02 |
| Fluorescence | Global | 20/80 | 7.32 ± 0.17 | 1.01 ± 0.02 | |
| HX | R16 (α1) | 20/80 | 7.67 ± 0.08 | 7.12 ± 0.03 | 0.82 ± 0.04 |
| L18 (α1) | 7.74 ± 0.13 | 7.17 ± 0.08 | 0.98 ± 0.06 | ||
| A20 (α1) | 8.22 ± 0.12 | 7.34 ± 0.04 | 1.05 ± 0.13 | ||
| I21 (α1) | 7.44 ± 0.18 | 7.58 ± 0.01 | 1.04 ± 0.08 | ||
| N27 (α1) | 8.81 ± 0.14 | 9.00 ± 0.17 | 1.05 ± 0.08 | ||
| F51 (α3) | 7.43 ± 0.07 | 7.26 ± 0.06 | 0.99 ± 0.06 | ||
| N52 (α3) | 7.41 ± 0.06 | 7.26 ± 0.04 | 0.79 ± 0.07 | ||
| A62 (α4) | 7.64 ± 0.12 | 6.86 ± 0.02 | 0.90 ± 0.08 | ||
| L65 (α4) | 7.65 ± 0.20 | 7.79 ± 0.02 | 1.07 ± 0.10 | ||
| K67 (α4) | 7.93 ± 0.10 | 7.27 ± 0.03 | 1.02 ± 0.03 | ||
| I68 (α4) | 8.18 ± 0.23 | 7.44 ± 0.02 | 1.14 ± 0.10 | ||
| V71 (α4) | 7.27 ± 0.10 | 6.97 ± 0.02 | 0.97 ± 0.05 |