Table S2.
ΔGHX at different urea and sarcosine concentrations
| Sarcosine | Urea | ||||||||||
| Site | 1 M | 0.5 M | 0 M | 0.5 M | 1 M | 1.5 M | 2.0 M | 2.5 M | 3.0 M | 3.5 M | 4.0 M |
| Global exchanging protons | |||||||||||
| R16 (α1) | 7.16(6) | 7.03(5) | 7.12(3) | 6.83(3) | 6.56(4) | 6.18(2) | 5.93(2) | 5.56(1) | 5.30(3) | 4.79(7) | 4.29(2) |
| L18 (α1) | 7.50(4) | 7.30(5) | 7.17(8) | 6.75(4) | 6.96(10) | 6.03(2) | 5.74(3) | 5.22(2) | 4.78(2) | 4.39(5) | 3.77(3) |
| A20 (α1) | 7.34(4) | 7.29(5) | 7.02(3) | 6.38(1) | 5.69(3) | 5.04(2) | |||||
| I21 (α1) | 8.32(2) | 7.58(1) | 6.01(1) | 5.94(1) | 5.10(3) | 5.13(3) | 4.42(2) | 3.79(6) | 3.25(2) | ||
| N27 (α1) | 9.02(7) | 9.04(9) | 9.00(17) | 7.91(8) | 7.76(2) | 7.04(10) | 6.51(8) | 6.35(11) | 5.68(9) | 5.40(5) | 4.47(2) |
| F51 (α3) | 7.48(3) | 7.05(4) | 7.26(6) | 6.80(1) | 6.30(3) | 5.75(2) | 5.29(3) | 5.12(1) | 4.42(1) | 3.99(8) | 3.43(4) |
| N52 (α3) | 7.48(11) | 7.26(4) | 6.70(3) | 6.53(1) | 6.10(4) | 5.86(4) | 5.55(3) | 4.98(1) | |||
| A62 (α4) | 6.93(6) | 6.61(2) | 6.86(2) | 6.57(5) | 6.30(3) | 5.93(1) | 5.76(3) | 5.52(2) | 5.00(2) | 3.93(2) | |
| L65 (α4) | 8.73(5) | 7.79(2) | 7.24(4) | 6.08(2) | 5.53(2) | 5.44(1) | 5.32(3) | 4.60(3) | 3.87(4) | 3.46(4) | |
| K67 (α4) | 7.49(8) | 7.31(7) | 7.27(3) | 7.07(2) | 6.72(3) | 6.25(2) | 5.78(3) | 5.47(3) | 4.88(2) | 3.80(5) | |
| I68 (α4) | 7.74(15) | 7.71(8) | 7.44(2) | 6.69(4) | 6.48(3) | 5.82(2) | 5.52(3) | 4.70(3) | 4.01(7) | 3.64(6) | |
| V71 (α4) | 9.04(10) | 7.91(6) | 6.97(3) | 6.65(2) | 6.46(5) | 5.69(3) | 5.19(4) | 5.06(2) | 4.39(4) | 3.90(2) | 3.32(3) |
| Nonglobal exchanging protons | |||||||||||
| E23 (α1) | 6.56(2) | 6.40(3) | 6.46(5) | 6.35(2) | 5.98(2) | 5.73(5) | 4.72(2) | 3.43(2) | |||
| K24 (α1) | 5.36(1) | 5.31(1) | 5.31(2) | 5.35(3) | 5.44(3) | 5.00(3) | 5.08(5) | 4.87(3) | 4.43(2) | 4.30(4) | 3.51(2) |
| K25 (α1) | 6.47(1) | 6.44(2) | 6.27(3) | 6.35(2) | 6.15(5) | 5.86(4) | 5.57(2) | 5.21(1) | 4.22(4) | ||
| K26 (α1) | 5.44(2) | 5.33(1) | 5.45(4) | 5.36(3) | 5.16(5) | 4.89(2) | 4.87(3) | 5.22(3) | 4.35(1) | 3.37(3) | |
| V36 (α2) | 5.02(1) | 4.88(1) | 5.10(1) | 4.88(3) | 4.76(2) | 4.51(1) | 4.54(2) | 4.36(2) | 4.02(2) | ||
| D38 (α2) | 4.27(7) | 4.28(4) | 4.34(3) | 4.38(2) | 4.18(1) | 3.97(2) | 4.30(1) | 4.12(3) | 3.59(1) | ||
| M40 (α2) | 5.98(1) | 5.82(3) | 5.82(3) | 5.73(1) | 5.69(1) | 5.37(1) | 5.21(4) | 5.11(6) | 4.71(2) | 4.68(8) | 3.79(3) |
| A48 (α3) | 5.01(2) | 4.82(3) | 4.91(2) | 4.88(1) | 4.74(3) | 4.56(2) | 4.69(3) | 4.37(2) | |||
| A49 (α3) | 5.21(2) | 5.53(4) | 5.24(2) | 5.09(3) | 5.45(4) | 5.28(3) | 5.34(1) | 5.05(3) | 4.68(3) | 4.59(3) | 3.83(2) |
| G53 (α3) | 5.36(2) | 5.21(1) | 5.19(1) | 5.17(5) | 5.10(5) | 4.95(4) | 4.95(1) | 4.67(1) | |||
| L69 (α4) | 5.26(4) | 5.23(1) | 5.04(1) | 4.86(5) | 4.64(3) | 4.72(2) | 4.02(2) | ||||
| E75 (310) | 7.01(5) | 6.77(4) | 6.70(1) | 6.49(3) | 6.05(4) | 5.77(1) | 5.51(2) | 5.34(1) | 4.70(2) | 4.27(3) | 3.59(2) |
| F76 (310) | 7.11(4) | 7.01(1) | 6.79(2) | 6.61(2) | 6.34(2) | 5.85(3) | 4.98(2) | 4.36(1) | |||
| R82 (α5) | 3.79(5) | 3.77(3) | 3.65(1) | 3.52(3) | 3.61(3) | ||||||
| E83 (α5) | 4.34(3) | 4.45(5) | 4.43(2) | 4.36(3) | 4.41(2) | 4.28(2) | 3.87(3) | ||||
Values in parentheses represent SEs of the fitting and derived parameters; e.g., 7.16(6) = 7.16 ± 0.06.