Table S3.
Location | Mutation | ψ0 | ϕbiHis, ϕ | ΔΔGeqZn2+ | ΔΔGmuteq | m0 | m0Zn2+ | βT = mf/mo | βTZn2+ |
WT(λYA) | — | — | — | 0 | 1.01 ± 0.02 | — | 0.76 ± 0.03 | — | |
α1 | A20H/K24H (biHis1) | 0.98 ± 0.28 | 0.63 ± 0.02 | 0.15 ± 0.06 | −1.21 ± 0.05 | 1.01 ± 0.02 | 0.85 ± 0.03 | 0.79 ± 0.02 | 0.71 ± 0.04 |
α2 | D38A | — | ND | — | 0.21 ± 0.05 | 1.05 ± 0.02 | — | 0.77 ± 0.03 | — |
D38G | — | 0.20 ± 0.09 | — | −0.55 ± 0.09 | 1.11 ± 0.04 | — | 0.71 ± 0.04 | — | |
A38G | 0.40 ± 0.05 | −0.74 ± 0.06 | 1.12 ± 0.03 | 0.75 ± 0.03 | |||||
α3 | S45H/A49H (biHis3) | 0.76 ± 0.06 | 0.55 ± 0.02 | 0.66 ± 0.06 | −1.29 ± 0.04 | 1.02 ± 0.02 | 1.07 ± 0.04 | 0.78 ± 0.02 | 0.81 ± 0.05 |
α4 | A63H/K67H (biHis4) | — | 0.66 ± 0.03 | — | −0.90 ± 0.05 | 1.02 ± 0.02 | — | 0.77 ± 0.02 | — |
A63G | — | 0.98 ± 0.07 | — | −0.43 ± 0.05 | 1.03 ± 0.02 | — | 0.75 ± 0.02 | — | |
α5 | S79H/E83H (biHis5) | 0.03 ± 0.02 | 0.30 ± 0.03 | 0.94 ± 0.07 | 0.79 ± 0.05 | 1.03 ± 0.02 | 1.19 ± 0.05 | 0.79 ± 0.02 | 0.88 ± 0.06 |