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. 2016 Apr 11;113(17):E2363–E2372. doi: 10.1073/pnas.1517066113

Fig. 2.

Fig. 2.

Evidence showing that the current recorded from TRPP2_F604P RNA-injected oocytes is conducted by the TRPP2_F604P channel. (A) Bar graph showing the dominant negative effect of WT TRPP2 on TRPP2_F604P current when both RNAs were coinjected in Xenopus oocytes. The relative amounts of the two RNAs injected are indicated at the bottom. Average currents at +60 mV are shown in this graph, and in C and D. (B) Applying a mouse anti-TRPP2 Ab that recognizes the extracellular loop of TRPP2 partially inhibited TRPP2_F604P current (Left), whereas applying a mouse anti-GFP Ab did not (Right). Ooctyes were clamped at −60 mV. (C) Bar graph showing the lack of GOF effect produced by F604A and F604I. Representative I-V curves and proteins’ surface expression are shown in Fig. S2. (D) Bar graph showing that two disease-causing mutations abolish TRPP2_F604P current. Representative I-V curves are shown in Fig. S2. Proteins’ surface expression is shown, together with other disease-causing mutations, in Fig. 5C. **P < 0.01; ***P < 0.001.