Fig. 3.

TRPP2_F604P current is inhibited by common cation channel blockers and divalent ions. (A) Bar graph showing that the TRPP2_F604P current was inhibited by adding Gd³⁺ (0.5 mM), amiloride (5 mM), or RuR (0.1 mM) into standard divalent ion-free bath solution. Average currents at −80 mV are shown. (B) Corresponding representative I-V curves for the data in A. (C and F) Bar graphs show the inhibition of TRPP2_F604P inward current by extracellular 2 mM Ca²⁺ (C) or 2 mM Mg²⁺ (F), and the effect of 5 mM EDTA (C). (D and G) Representative I-V curves of TRPP2_F604P in divalent ion-free bath solution (labeled as “None”) and solutions with 2 mM Ca²⁺ (D), 5 mM EDTA (D), or 2 mM Mg²⁺ (G) added are shown. (E and H) Concentration-dependent inhibition of inward (at −80 mV, orange traces) and outward (at +60 mV, blue traces) currents of the TRPP2_F604P by extracellular Ca²⁺ (E) and Mg²⁺ (H). Best-fit exponential curves are shown (n = 5 in both cases). Representative I-V curves under different concentration of Ca²⁺ are shown in Fig. S3A. **P < 0.01; ***P < 0.001.