Fig. S3.

Blocking of the TRPP2_F604P current. (A) Representative I-V curves of TRPP2_F604P when the indicated concentration of Ca²⁺ exists in bath solution. (B) Representative I-V curves showing the inhibition of 0.1 mM RuR on TRPP2_F604P current in the presence of 2 mM Ca²⁺. (C and D) The current size of TRPP2_F604P did not change when NaCl in bath solution was replaced by Na-gluconate in the presence of 2 mM Ca²⁺, as seen in the bar graph (C) and representative I-V curves (D). Bath solutions contain 2 mM Hepes-NaOH (pH 7.5) and indicated salts. The bar graph in C shows the outward current of TRPP2_F604P at +60 mV. (E) Comparison of the I-V curves of TRPP2_F604P in the presence of 2 mM Ca²⁺ and 2 mM Ba²⁺ showing the stronger blocking effect of Ba²⁺, especially on the outward current.