Fig. S4.

Effect of pH on TRPP2_F604P current. (A) I-V curves of TRPP2_F604P showing that pH has no significant effect on Ca²⁺ blocking. Curves are shown as the average of data from five oocytes (Left) and a representative oocyte (Right). Currents were recorded in the bath solution containing 100 mM NaCl, 2 mM CaCl₂, and 2 mM Hepes. The pH was adjusted with NaOH or HCl. (B) I-V curves showing that low pH inhibits the current of TRPP2_F604P in the absence of Ca²⁺. Curves are shown as the average of data from five oocytes (Left) and a representative oocyte (Right). Currents were recorded in the bath solution containing 100 mM NaCl and 2 mM Hepes. The pH was adjusted to 4 with HCl.