Fig. S8.

Phenotype rescue and TRPP2 expression in zebrafish. (A) Bar graph comparing the percentages of fish with the normal tail phenotype in the groups that were injected with TRPP2 MO only or the combinations of MO and the indicated RNA. A significant difference is seen between the group injected with MO + WT TRPP2 RNA and the group injected with MO + TRPP2_F604P RNA. Data were collected in five independent experiments and analyzed with Student's t test. *P < 0.05. (B) Overall TRPP2 protein expression in whole zebrafish at 48 hpf. RNA of human TRPP2, TRPP2_F604P, or TRPP2_F604P_D511V was injected into zebrafish at the one-cell stage. Mouse monoclonal anti-HA Ab was used to detect HA-tagged human TRPP2 proteins.