Figure 2.

Fatty livers are more sensitive to I/R injury, both in vivo and in vitro. (a) Lean and ob/ob mice subjected to 1 h of liver ischemia and 24 h of reperfusion or sham operated. Liver histology images of the area of focal necrosis are shown (magnification × 200). Quantification of the necrotic areas is shown (right; n=4 per group). (b) After 24 h of reperfusion, TUNEL-positive cells were detected in lean livers and significantly increased in ob/ob mice livers. (c) Serum was obtained at 6 h of reperfusion and ALT levels were determined. (d and e) Isolated hepatocytes from lean and ob/ob mice underwent 4 h of anoxia and 2 h of reoxygenation. PI (red spots, d) and TUNEL (green spots, e) positive cells were calculated at each time point of reoxygenation. Cell nuclei were stained by Hoechst 33342 (blue spots). (f) Hepatocellular ATP concentration was determined at the indicated time points. ^#P<0.05 versus the sham or 0 h of anoxia, *P<0.05 versus the lean group