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. 2016 Apr 7;7(4):e2174. doi: 10.1038/cddis.2016.73

Figure 1.

Figure 1

KCa3.1 inhibition modifies the polarization of CD11b+ cells extracted from glioma-bearing mice and from biopsies of human GBM. (a) RT-PCR of M2-like (arg1, ym1, fizz1, cd163 and cd206) and M1-like (cd86, tnfα, inos, il1β and il6) related genes in CD11b+cells extracted from ipsilateral and contralateral cerebral hemispheres of GL261-bearing mice treated with vehicle (C) or TRAM-34 (T, 120 mg/kg/die). Data are the mean±S.E.M., *P<0.05 versus C contralateral; #P<0.05 versus C ipsilateral by two-way ANOVA; N=8. (b) Tumor volumes in the brain of mice treated with vehicle or TRAM-34. Data are the mean±S.E.M., *P<0.05 by Student's t-test; N=8. Scale bar: 1 mm. (c) RT-PCR for human M2-like (CD163, MMP12, TGFB1) and M1-like (CXCL10, IL12A, NOS2, TNF) related genes expressed by CD11b+ cells extracted from human GBM specimens and treated for 24 h with TRAM-34 (2.5 μM). Data are expressed as fold change of TRAM-34-treated versus untreated samples and are the mean±S.E.M., *P<0.05 by Student's t-test; N=4