Figure 1.

Tak1 deletion depletes Paneth cells. (a) H&E staining (upper panels) and immunofluorescence staining of Paneth cell marker, lysozyme (red) (lower panels) of control and the non-inducible version of intestinal epithelial-specific Tak1-deficient ileum on a Tnfr1^−/− background at postnatal day 17. Scale bars, 20 μm. See also Supplementary Figures S1A–C. (b) H&E staining of the crypts of ileum (3 months old). Scale bars; upper panels, 20 μm; lower panels (high magnifications), 10 μm. (c) H&E staining of control and the inducible version of intestinal epithelial-specific Tak1-deficient crypts of ileum on a Tnfr1^−/− background. Tamoxifen was injected for three consecutive days and analyzed at 4, 7 or 2 months after the tamoxifen treatment. Black arrows indicate structurally disrupted Paneth cells. Black scale bars, 20 μm: red scale bars, 10 μm. See also Supplementary Figures S1D–E