Figure 2.

Tak1 deletion depletes Paneth cells without altering cell proliferation. (a) Alcian blue staining of no-Cre Tnfr1^−/− and Tak1^IE-IKO Tnfr1^−/− at day 3 (upper panels) or 7 (lower panels) after tamoxifen injection. To visualize weak staining of Alcian blue, no counterstaining was performed. Goblet cells were observed in lower magnification images (lower panels). Red arrows indicate the bottom of crypts. Scale bars, 50 μm (upper panels); 200 μm (lower panels). (b) No-Cre Tnfr1^−/− and Tak1^IE-IKO Tnfr1^−/− at day 14 after tamoxifen injection were treated with EdU for 4 h. EdU and DAPI staining are shown. Scale bars, 50 μm. Red arrows indicate the bottom of crypts