Figure 4.

Myd88 deletion reduces ROS and apoptosis. (a) ROS-sensitive dye staining of Myd88 heterozygous or Myd88 homozygous deletion Tak1^IE-IKO Tnfr1^−/− ileum crypts at days 10–12 after tamoxifen injection. Scale bars, 50 μm. (b) ROS-positive cells in each crypt of samples in (a) were counted (more than 100 crypts per mouse). Myd88 heterozygous deletion Tak1^IE-IKO Tnfr1^−/− mice; n=7; Myd88 homozygous deletion Tak1^IE-IKO Tnfr1^−/− mice; n=8. All data points are shown in the box and whisker plots: median and distribution of 50% of values are shown in the box: whiskers indicate distribution of minimum and maximum values. **P<0.01 (two-tailed unpaired Student's t-test). (c) TUNEL staining. Scale bars, 50 μm. (d) Quantification of TUNEL-positive staining (more than 30 crypts per mouse). Myd88 heterozygous deletion Tak1^IE-IKO Tnfr1^−/− mice; n=3; Myd88 homozygous deletion Tak1^IE-IKO Tnfr1^−/− mice; n=3. Means±S.E.M., *P<0.05 (two-tailed unpaired Student's t-test)