Fig. 4.

Expression of paired Trem genes in BMDCs. a Paired Trems expression after TLR agonist stimulations in BMDCs. Concentrations of stimulants used were as follows: Pam3CSK4, 5 μg/ml; polyI:C (pIC), 20 μg/ml; LPS, 0.1 μg/ml; Flagellin A (FlaA), 0.3 μg/ml; CpG, 2.5 μg/ml with DOTAP. b Type I IFN production in Wild-type, Ticam-1 ^−/− and Mavs ^−/− BMDCs. BMDCs prepared from each mouse line were stimulated with pIC (20 μg/ml). 24 h later, the mounts of IFNα and IFNβ in culture supernatants were measured by ELISA. c Treml6 and Trem4 mRNA expressions after pIC stimulation in KO mice-derived BMDCs. Wild-type, Ticam-1−/−, Mavs−/− and Ifnar1−/− derived BMDCs were stimulated by pIC (20 μg/ml) for 12 h. d Type I IFN signal is indispensable for Treml6 and Trem4 expression of BMDCs derived from Wild-type mice. BMDCs were stimulated with pIC (20 μg/ml) and anti-IFN receptor blocking antibody or isotype control (10 μg/ml) for 12 h. In these experiments, mRNA expressions of paired Trem genes were measured by qPCR and their expression levels were normalized to that of Gapdh mRNA. Data are representative of three independent experiments. The data shown are representative of at least two independent experiments. Data are means ± SD of three independent samples. *p < 0.05. **p < 0.01