Additional file 4: Video 1.
Premature cell death of persistent muscles. Overexpression of dominant negative TOR (TOR TED), and RNAi of Rm62 and Cp1 induce histolysis of DIOMs, which persist throughout metamorphosis until adulthood in controls (top left). The time-lapse frames show the development from the prepupal (−6 h) to the late pupal stage (+100 h). Muscles are labelled with tau-GFP (green) and histone-mKO (red). Pupae are oriented in an anterior–posterior direction from left to right. Views show part of the thorax and abdominal segments A1 to A4. Note the stability of fluorescently labelled sarcolytes derived from histolyzed muscles. None of the gene perturbations caused obvious cell death of newly formed adult muscles, such as IFMs, heart and abdominal muscles. The time-lapse images were recorded at 22 °C. (MP4 8845 kb)