Additional file 5: Video 2.

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Silencing of AMPKα leads to loss of tubular morphology and disintegration of DIOMs. Compared to control muscles (left panel), AMPKα ^shRNA DIOMs gradually lose tubular morphology from +15 h aHE onwards. From around +30 h, DIOMs turn into floating muscle spheroids, indicating a loss of cell attachment. Later, green fluorescence of spheroids rapidly disappears within 30 min intervals (see arrow at +56:30 h), while condensed nuclei stay behind, indicating rapid lysis without formation of sarcolytes. The time-lapse images were recorded at 25 °C. Muscles were labeled with Mhc-tau-GFP (green) and histone-mKO (magenta). (MP4 6582 kb)