. 2016 May 4;16:12. doi: 10.1186/s12861-016-0113-1

Additional file 6: Video 3.

Silencing of Atrophin inhibits muscle histolysis in metamorphosis. Atro-RNAi (right panel) delays histolysis of DEOM1s in all abdominal segments. While control DEOMs (left panels) are destroyed at the onset of HE, Atro ^shRNA muscles persist for up to 10 h into pupation. In abdominal segment A2, DEOMs (arrow) survive until adulthood. The time-lapse images were recorded at 25 °C. Muscles were labeled with Mhc-tau-GFP (green) and histone-mKO (magenta). The scale bars correspond to 200 μm. (MP4 8280 kb)