Table 1.
List of gene perturbations that affect survival of larval abdominal muscles in metamorphosis
| Gene/synonym | gene pertur-bation | Stock ID/TRiP # | Muscle phenotype | Flightless |
|---|---|---|---|---|
| Chromator (Chro), control | RNAi | B-36084/GL00503 | wildtype | no |
| Rm62 | RNAi | B-34829/HMS00144 | DIOM cell death | yes |
| Cysteine Proteinase 1 (Cp1)/Cathepsin L | RNAi | B-32932/HMS00725 | DIOM cell death | no |
| Cysteine Proteinase 1 (Cp1)/Cathepsin L | RNAi | B-41939/HMS02336 | DIOM cell death | no |
| TOR/TOR-TED | DN | B-7013 | DIOM cell death, delay of DEOM cell death | partial |
| AMP-activated protein kinase α (AMPKα)/SNF1a | RNAi | B-35137/GL00004 | DIOM loss of morphology and degeneration | yes |
| Atrophin (Atro)/Grunge | RNAi | B-32961/HMS00756 | Inhibition of DEOM cell death | yes |
Initial phenotypic assessment of a minimum of 20 pupae per genotype was carried out using a macro zoom microscope