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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2015 May 31;1853(10 Pt A):2404–2410. doi: 10.1016/j.bbamcr.2015.05.032

Figure 4. Western blot and NADPH oxidase activity in mesenteric resistance arteries (n=4).

Figure 4

(A) Western blot analysis showing the phosphorylated (P) and total (T)-eNOS, Akt, phosphorylated (P)-ERK1/2, Rho Kinase, p22phox and p47phox expression in MRA from control mice incubated with exogenous EGF in the presence or absence of gp91 ds-tat; (B) Western blot analysis showing phosphorylated-Rho Kinase in MRA from db−/db− mice incubated with exogenous EGF in the presence or absence of gp91 ds-tat; (C) Western blot analysis showing the total and phosphorylated-eNOS, p22phox expression in MRA from control and db−/db− mice incubated with AG1478.

NADPH oxidase activity (D), RT PCR for NOX2 and NOX4 (E, F) in lysates of mesenteric resistance arteries from control mice incubated with exogenous EGF in the presence or absence of gp91 ds-tat; *p< 0.05 for control EGF vs. control and control EGF + gp91 ds-tat.