Table 1.
Pentatricopeptide (PPR) and tetratricopeptide (TPR) repeat containing proteins identified in affinity-purified large ribosomal subunit L3 and small ribosomal subunit S5 and S17 protein samples.
| Gene ID | Name | Motif | L3-PF | L3-BF | S17-PF | S5-BF |
|---|---|---|---|---|---|---|
| Tb927.7.3460 | KRIT2 | TPR | 1235 | 637 | 418 | |
| Tb927.10.380 | KRIPP5 | PPR | 1159 | 504 | 227 | |
| Tb927.8.3170 | KRIPP9 | PPR | 1040 | 564 | 232 | |
| Tb927.11.5500 | KRIPP1 | PPR | 980 | 1254 | 485 | |
| Tb927.6.3930 | KRIT1 | TPR | 935 | 549 | 224 | |
| Tb927.11.11470 | KRIPP14 | PPR | 625 | 844 | 375 | |
| Tb927.3.4550 | KRIPP6 | PPR | 561 | 254 | ||
| Tb927.3.5240 | KRIPP8 | PPR | 556 | 961 | 541 | |
| Tb927.1.1160 | KRIPP3 | PPR | 496 | 188 | 171 | |
| Tb927.4.2790 | KRIPP12 | PPR | 494 | 318 | ||
| Tb927.9.5280 | KRIPP16 | PPR | 470 | 815 | 376 | |
| Tb927.9.12750 | KRIT3 | TPR | 389 | |||
| Tb927.2.4460 | KRIPP21 | PPR | 325 | 222 | ||
| Tb927.4.4720 | KRIPP7 | PPR | 305 | 322 | ||
| Tb927.11.4650 | KRIPP13 | PPR | 280 | 101 | ||
| Tb927.1.2990 | KRIPP2 | PPR | 267 | 638 | 293 | |
| Tb927.11.7680 | KRIPP22 | PPR | 241 | 301 | ||
| Tb927.8.4860 | KRIPP10 | PPR | 144 | 562 | 248 | |
| Tb927.10.11820 | KRIPP17 | PPR | 103 | 641 | 205 | |
| Tb927.7.2490 | KRIPP15 | PPR | 263 | |||
| Tb927.6.610 | KRIPP18 | PPR | 385 | |||
| Tb927.10.13200 | KRIPP4 | PPR | 274 | |||
| Tb927.8.6040 | KRIPP11 | PPR | 967 | 149 | ||
| Tb927.3.5610 | L3 | L3 ribo prot | 1855 | 600 | 182 | |
| Tb927.9.11280 | S17 | S17 ribo prot | 562 | 197 | 1685 | 337 |
| Tb927.10.6300 | S5 | S5 ribo prot | 640 | 1080 | 1300 |
Respective TAP-tagged proteins (in bold) were overexpressed in procyclic (PF) and bloodstream (BF) parasites. SAF values for each polypeptide are provided for each preparation. Previously, identified KRIPP19 and KRIPP20 (Aphasizheva et al., 2011) were excluded under cutoff implemented in this study (SAF < 100). Conversely, association of additional PPR (KRIPP22) and TPR (KRIT3) proteins with the large ribosomal subunit has been detected. KRIPP, kinetoplast ribosome-associated PPR protein; KRIT, kinetoplast associated TPR protein. Motifs are from Pfam.