Table 1. Dynamics of clathrin adaptors, phosphoinositide binding reporters, and Golgi markers at the TGN.

Haploid cells expressing the indicated fluorescent proteins were imaged using live-cell microscopy. Multiple puncta from each strain were analyzed for changes in fluorescent intensity of the indicated proteins over time. Time between the points of peak fluorescence intensity for each protein was determined and the mean peak-to-peak time calculated for all the analyzed puncta in a given strain. The data are presented as the mean ±S.E.M. for each strain. The order of tagged proteins in each row represents the order of assembly. Some rows are repeated to facilitate comparison (1,15,24; 4,26; 8,13; 17,22).

#	Strain		Number of puncta (n) in total cells (c)		Peak to Peak time (seconds)
	mutations	labeled proteins	n	c
1	wt	Gga2-mRFP β1-GFP	102	59	10.4 ± 0.55
2	wt	Gga2-mRFP Ent3-GFP	91	38	0.01 ± 0.16
3	wt	Ent3-GFP β1-mRFP	30	21	10.2 ± 1.40
4	wt	Ent3-GFP Ent5-mRFP	99	52	8.4 ± 0.70
5	wt	Gga2-mRFP Ent5-GFP	91	46	8.0 ± 0.14
6	wt	Ent5-GFP β1-mRFP	58	30	1.3 ± 0.62
7	wt	Gga2-GFP Sec7-mRFP	97	25	0.29 ±0.39
8	wt	Sec7-mRFP β1-GFP	76	48	10.4 ± 1.18
9	wt	Gga2-GFP Chc1-mRFP	95	49	1.3 ± 0.4
10	wt	Chc1-mRFP β1-GFP	59	34	8.0 ± 0.7
11	gga1Δ gga2Δ	Chc1-mRFP β1-GFP	71	36	0.79 ± 0.73
12	β1Δ (apl2Δ)	Chc1-mRFP Gga2-GFP	22	40	0.9 ±0.64
13	wt	Sec7-mRFP β1-GFP	76	48	10.4 ± 1.18
14	gga1Δ gga2Δ	Sec7-mRFP β1-GFP	49	25	26.1 ± 1.9
15	wt	Gga2-mRFP β1-GFP	102	59	10.4 ± 0.55
16	arf1Δ	Gga2-mRFP β1-GFP	47	41	20.5 ± 2.7
17	wt	Gga2-mRFP GFP-PHOSH1	104	52	5.3 ± 0.44
18	wt	GFP-PHOSH1 β1-mRFP	87	52	4.1 ± 0.4
19	wt	Sec7-mRFP GFP-PHOSH1	103	61	3.8 ± 0.54
20	gga1Δ gga2Δ	Sec7-mRFP GFP-PHOSH1	117	68	11.3 ± 0.86
21	arf1Δ	Sec7-mRFP GFP-PHOSH1	45	33	11.0 ± 2.1
22	wt	Gga2-mRFP GFP-PHOSH1	104	52	5.3 ± 0.6
23	GPD-PIK1 GPD-FRQ1	Gga2-mRFP GFP-PHOSH1	48	27	0.0 ±0.81
24	wt	Gga2-mRFP β1-GFP	102	59	10.4 ± 0.55
25	GPD-PIK1 GPD-FRQ1	Gga2-mRFP β1-GFP	67	35	5.7 ±0.77
26	wt	Ent3-GFP Ent5-mRFP	99	52	8.4 ± 0.70
27	GPD-PIK1 GPD-FRQ1	Ent3-GFP Ent5-mRFP	30	17	3.9 ±1.5
28	wt	Sec7-mRFP GFP-Pik1	75	47	4.1 ± 0.6
29	gga1Δ gga2Δ	Sec7-mRFP GFP-Pik1	61	36	12.6 ± 1.3
30	wt	Frq1-GFP Sec7-mRFP	55	38	1.5 ± 0.8
31	gga1Δ gga2Δ	Frq1-GFP Sec7-mRFP	73	48	0.4 ± 0.9