Fig. 5.

MiR-300 and miR-609 are associated with inhibition of SMAD4 gene expression. a Relative expression of SMAD2, SMAD3, and SMAD4 were evaluated by qRT-PCR for SjS (n = 12) and HC (n = 10) CD14⁺ monocytes. Data represents log2-transformed gene expression. ^** P < 0.01, ^*** P < 0.001 by two-tailed t test. b Target prediction of SMAD4 3′UTR by miR-300 and miR-609 represents target-miRNA alignments with the highest predicted minimum free energy (MFE). Position of alternative polyadenylation sites are indicated in the SMAD4 3′UTR. c Linear regression analyses of miR-300 and miR-609 with SMAD4 gene expression. HC healthy control, pSjS primary Sjögren’s syndrome, SjS Sjögren’s syndrome