Figure 6. APP, APP mutants, or C99 inhibited NGF-induced neurite outgrowth that was rescued by Rab5^S34N.

PC12M cells were transfected with the indicated plasmids and treated with 50 ng/ml NGF for 48 hours. The length and the number of neurites of each cell were measured and quantitated using ImageJ. Representative images are shown in A. The number of the neurites/cell (B) and the average length (C) in each condition are measured, and the results are shown. (D–F) Rab5^S34N-GFP or Rab5^S34N-mCherry was cotransfected with APP-mCherry or Rabex-5–GFP into PC12M cells (D). Cells were then induced for differentiation. The number of the neurites/cell (E) and the average length (F) of each condition were measured, and the results are shown. All P values were calculated using Student’s t test; ***P < 0.001. Scale bars: 20 μm.