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. 2016 Apr 18;126(5):1939–1952. doi: 10.1172/JCI83658

Figure 2. Kidneys are not protected from IRI when VNS is applied 10 minutes before IRI.

Figure 2

Mice underwent left VNS (5 Hz, 1 ms, 50 μA for 10 minutes) or sham stimulation surgery 10 minutes prior to IRI (26 minutes ischemia, 24 hours reperfusion) or sham IRI surgery. VNS applied 10 minutes before IRI did not protect kidneys from IRI, as shown by plasma creatinine (A), tissue morphology (B; representative H&E staining of kidney sections), and ATN (C; scored from H&E samples). n = 4 each. Data were analyzed using 1-way ANOVA. Means were compared by post hoc multiple-comparison test (Tukey’s). ***P < 0.001. Scale bars: 100 μm; 50 μm (inset).