Table 2.
Growth kinetics and stoichiometry of P. putida KT2440 under different growth-limiting conditions
| Limitation | D (h−1) | Residual ammonium (mg L−1) | Residual glycerol (g L−1) | YX/S (g g−1) | PHA (wt%) | YPHA/S (g g−1) | Uptake and production rates (mmol gCDW−1 h−1) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Glycerol | Malate | Succinate | CO2 | |||||||
| Glycerol | 0.044 | 68.2 ± 4.8 | ND < 0.1 | 0.47 | 2.5 | – | 1.02 ± 0.10 | ND < 0.1 | ND < 0.1 | 1.5 ± 0.1 |
| Ammonium | 0.044 | ND < 0.1 | 19.9 ± 0.9 | 0.19 | 29.7 | 0.08 | 2.47 ± 0.15 | 0.04 ± 0.01 | 0.24 ± 0.02 | 3.6 ± 0.1 |
| Glycerol | 0.120 | 35.1 ± 2.7 | ND < 0.1 | 0.51 | 2.7 | – | 2.60 ± 0.06 | ND < 0.1 | ND < 0.1 | 3.2 ± 0.1 |
| Ammonium | 0.120 | ND < 0.1 | 25.7 ± 0.7 | 0.34 | 12.8 | 0.05 | 3.71 ± 0.03 | 0.21 ± 0.03 | ND < 0.1 | 4.2 ± 0.1 |
Standard deviation (±) from at least two independent experiments
Yx/s was calculated based on the biomass, excluding PHA
ND not detected in the culture broth