Design of a 24-subunit protein cube. (a) The designed fusion protein with the KDPGal aldolase trimer (green) connected to the dimeric domain of FkpA protein (orange) by the helical linker (blue). The purple and cyan lines represent the two-fold and three-fold axes of symmetry, respectively. (b) A cartoon model of the 24-subunit cage design. The two-fold and three-fold axes of symmetry in the cube are shown in purple and cyan, respectively. Reprinted by permission from Macmillan Publishers Ltd: [Nature Chemistry] Lai, Y. T.; Reading, E.; Hura, G. L.; Tsai, K. L.; Laganowsky, A.; Asturias, F. J.; Tainer, J. A.; Robinson, C. V.; Yeates, T. O. Nat Chem 2014, 6, 1065–1071, © 2014.