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Neo-N3 inhibits Drosha processing of pri-miR-525 in cell extracts. Note: cell extracts have endogenous Dicer activity, enabling processing to the mature miRNA. Left, representative gel image of pri-miR-525 processing in the presence and absence of Neo-N3. Right, quantification of processing as a function of Neo-N3 concentration. Data were normalized for the number of A residues in each product, as the substrate was internally labeled.
