Table 1. Primers used for real-time quantitative RT-PCR and polymorphism analyses and plasmid construction of chicken MMP13 gene.
| Primer Name | Sequence (5′–3′) | Annealing Temperature (°C) | Size (bp) |
|---|---|---|---|
| MMP13-F | TTTGGATTAGAGGTGACGG | 56 | 253 |
| MMP13-R | CCACTTCGTATTCTGGTGA | ||
| β-actin-F | TGGATGATGATATTGCTGC | 56 | 251 |
| β-actin-R | ATCTTCTCCATATCATCCC | ||
| VEGFA-F | GGAAGCCCAACGAAGTTA | 55 | 231 |
| VEGFA-R | CGCTATGTGCTGACTCTGAT | ||
| snpMMP13-F | ACAATCCCAGTTCCCTCAG | 58 | 807 |
| snpMMP13-R | GCCCAGGCTTGTATCACT | ||
| pGL3-MMP13-1F | CGACGCGTGTGCTGTGCTGTAAGTTGTCTT | ||
| pGL3-MMP13-2F | CGACGCGT CACTTCTCCCATTCTTCCAT | ||
| pGL3-MMP13-3F | CGACGCGT ACAATCCCAGTTCCCTCAG | ||
| pGL3-MMP13-4F | CGACGCGT AGTGATACAAGCCTGGGC | ||
| pGL3-MMP13-5F | CGACGCGT GTCATGTCCTATTATGTACTCC | ||
| pGL3-MMP13-R | GGAGATCTTTTGTGAAGTCTGAAGCCC | ||
All forward primers contained an MluI site at their 5′-ends, and the reverse primers had an added BglII site at their 5′-ends (underlined).