Figure 2.

Functional characterization of the mutant strain Δseb-1. (A) 10-day-old conidia of the Δseb-1 strain were cultivated in Erlenmeyer flasks and on plates containing solid VM medium. The images of the hyphae tips were captured after 24 hr using an AxioCam ICc3 camera coupled to a trinocular Discovery V8 stereoscope (Zeiss) at 80× magnification. (B) Heat stress assay. The Δseb-1 strain was cultivated at 45° for 2 d and then brought back to 30° for 24 hr to evaluate cellular viability. Cultures at 30° for 24 hr are shown as control. The Δseb-1 seb-1⁺ strain is the Δseb-1 complemented strain (Δseb-1 his-3::Pccg-1-seb1-sfgfp). VM, Vogel’s minimal; WT, wild-type.