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. 2016 Mar 16;6(5):1327–1343. doi: 10.1534/g3.116.028506

Figure 3.

Figure 3

SEB-1 protein translocates to the nucleus during heat stress. Conidia from the Δseb-1 complemented strain (Δseb-1 his-3::Pccg-1-seb1-sfgfp) were grown on coverslips in liquid VM medium containing 1% sucrose at 30° for 10 hr. After this period, mycelia were transferred to VM medium preheated at 45° (heat stress) for 30 min, 1 hr, and 2 hr. Heat-shocked mycelia were fixed in PBS with formaldehyde, the nuclei were stained with DAPI (0.5 mg/ml), and the fluorescence was examined. Mycelium cultured in liquid VM medium at 30° for 10 hr was used as a control. Images were taken after 30 min, 1 hr and 2 hr after transferring to 45°. Fluorescence was evaluated using the AXIO Imager.A2 microscope (Zeiss) at a magnification of 100×. The images are representative of at least two independent experiments. Scale bar: 10 μm. DAPI, 4’,6-diamino-2-phenylindole; GFP, green fluorescent protein; PBS, phosphate-buffered saline; VM, Vogel’s minimal.