Figure 6.

The Δseb-1 strain exhibits impaired accumulation of reserve carbohydrates during heat stress. Liquid cultures of the WT, Δseb-1, and Δseb-1 complemented strains were exposed to a temperature shift from 30° to 45° for 30 min. After this, the heat-shocked mycelia were used to prepare a cellular extract used for glycogen and trehalose quantification, as described in the Materials and Methods. (A) Glycogen content. (B) Trehalose content. WT: wild-type strain; Δseb-1: strain mutated in the ORF NCU02671; Δseb-1 seb-1⁺: Δseb-1 complemented strain (Δseb-1 his-3::Pccg-1-seb1-sfgfp). The error bars represent the standard deviation. Values of three biological replicates were used for statistical analysis and the significances (*P < 0.05, **P < 0.01) between the strains were estimated by the Tukey-Kramer multiple comparison test.