Figure 4.

Quantitative assessment of eye phenotypes due to gene-dosage alteration. (A) Plot of mean values of gene expression of RNAi lines with eye specific knockdown at 28° vs. 30°. Note that each experiment was conducted in triplicate, and then repeated using a fresh preparation of RNA and cDNA synthesis. Gene expression of flies reared at 30° is lower than that at 28° due to increased RNAi-mediated knockdown at 30° (note that most red circles are below the black diagonal line), indicating a temperature dependent effect of the UAS-Gal4 system. (B) A graph representing phenotypic scores for knockdown of prosap, dube3a, para, pten, arm, caps, and kismet at 30° and 28° is shown. A dosage dependent increase in severity was observed for all the genes tested. Asterisks (*) show significant P-values (student t-test, corrected two-tailed P < 0.001) when phenotypic scores at 30° was compared to that at 28°. The number of images processed for each genotype ranged from n = 14 to n = 30. A complete list of statistical analysis and n numbers for each of the genotype assessed is presented in Table S6.